A new program was launched to generate a resource comprising most of the secreted proteins in humans. The genes coding for the proteins predicted to be secreted were constructed with synthetic biology and used for mammalian bioproduction using Chinese hamster ovary (CHO) cells. The program was initiated by a collaboration involving the Wallenberg Foundation, the Novo Nordisk Foundation, and the pharmaceutical company AstraZeneca.
Key publication
- Tegel H et al., High throughput generation of a resource of the human secretome in mammalian cells. N Biotechnol. (2020)
PubMed: 32502629 DOI: 10.1016/j.nbt.2020.05.002
Other selected publications
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Uhlén M et al., The human secretome. Sci Signal. (2019)
PubMed: 31772123 DOI: 10.1126/scisignal.aaz0274
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Jennbacken K et al., Phenotypic Screen with the Human Secretome Identifies FGF16 as Inducing Proliferation of iPSC-Derived Cardiac Progenitor Cells. Int J Mol Sci. (2019)
PubMed: 31801200 DOI: 10.3390/ijms20236037
Figure legend: Outline of the CHO mammalian cell factory for generation of a resource of the human secretome.
Key facts
- More than 3,000 full-length genes have been generated with synthetic biology
- More than 1,500 proteins have been produced and recovered from the CHO culture medium
- The human secretome resource has been used for phenotypic assays in various screening platforms
The Human Protein Atlas project is funded
