Methods

The Human Protein Atlas consists of 12 sections. In the following, explore the Methods Summary of respective section.

Technologies

Antibodies are used in a variety of research applications and techniques, such as Western Blot, Enzyme-Linked Immunosorbent Assay, multiplex and singleplex Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, and more. The different conditions in each application and the complexity of biological samples ask for great functionality from the antibodies.

The following pages describe some of the most common technologies.


Antibodypedia

Enzyme-linked immunosorbent assay

Flow cytometry

Immunocytochemistry

Immunoelectron microscopy

Immunohistochemistry

Multiplex immunohistochemistry

Immunoprecipitation

Immunoproteomics

Proximity assays

Western blot

References and Links

Relevant Wikipedia pages:
http://en.wikipedia.org/wiki/Antibodies
http://en.wikipedia.org/wiki/Polyclonal_antibodies
http://en.wikipedia.org/wiki/Monoclonal_antibody

Antibodypedia - An open-access database of publicly available antibodies and their usefulness in various applications:
http://www.antibodypedia.com