Subcellular - RAB5C - The Human Protein Atlas

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RAB5C

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Field

Cluster

Pathway

SUMMARY

TISSUE

BRAIN

SINGLE CELL

TISSUE CELL

PATHOLOGY

DISEASE

IMMUNE

BLOOD

SUBCELL

CELL LINE

STRUCTURE

INTERACTION

RAB5C

Please wait until image is loaded before changing channels

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RAB5C
Gene descriptionⁱ Full gene name according to HGNC.	RAB5C, member RAS oncogene family
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s).	Enzymes Plasma proteins RAS pathway related proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	7

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Main locationⁱ Main subcellular location(s) and reliability score(s) for the encoded protein(s) in human cells. The main location(s) may be characterized by presence in all tested cell lines and/or ihigher staining intensity compared to the potential additional location(s). If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.	Localized to the Endosomes (enhanced)
GFPⁱ Validation score for the subcellular location(s) of the encoded proteins(s) according to a GFP assay.	The location(s) are Enhanced based on analysis of GFP-tagged protein.
Reliability scoreⁱ Overall gene reliability score for the subcellular location(s) of the encoded protein(s). The score indicates the level of reliability of the annotated protein localization based on agreement with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database and presence of antibody validation assays for immunocytochemistry.	Enhanced
Antibodiesⁱ Antibodies used for this assay. Click on an antibody for more information.	HPA004167
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ASSAYSⁱ

RNA expression

Human cells

Custom data

GFP

RNA EXPRESSION OVERVIEWⁱ

Organ

Origin

Category

Expression

Alphabetical

Cell lines ordered by organ of phenotypic resemblance.

HUMAN CELLSⁱ

Summaryⁱ

Localized to the endosomes.

Main locationⁱ

Endosomes (enhanced)


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel. The object toggle shows the computational segmentation of the cells used for further analysis in the HPA project. For samples where cell cycle dependency for the protein is suggested according to a correlation assay the predicted cell cycle position of each cell is displayed when using the object toggle.	Low High

Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.	Cell line RNA Expression (nTPM)ⁱ RNA expression level in the cell line based on normalised RNA sequencing data. Genes with a value above 1 NX are considered to be expressed.	Locationⁱ Observed subcellular location(s).
HPA004167	A-549	276.3	No staining
HPA004167	HEK293	218.2	Endosomes
HPA004167	U2OS	158.8	Endosomes

CUSTOM DATAⁱ


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images.

Thumbnailⁱ Minatures of all available images. The checkboxes can be used to select which three images to compare in the window above. All images are also clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay.	Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.	Assayⁱ Depending on the type of custom data the assay can be an immunofluorescent image, an immunofluorescent image stack, siRNA validation data or a time-lapse movie.	Descriptionⁱ Description of the assay used to generate the displayed custom data.
	HPA004167	U2OS	Image	RAB5C localizes to endosomes, as it partially co-localizes with the endosomal marker EEA1.

GFPⁱ

Antibodyⁱ

HPA004167

Validationⁱ

N-terminal tag
BAC cell line: 5950
Enhanced: Antibody staining overlaps with GFP tagged protein


	Toggle channelsⁱ Buttons for turning on and off the different channels in the multi-color images. The HPA-antibody staining targeting the protein of interest is shown in green and the staining of the GFP-tagged protein in purple. The intensity toggle shows the pixel intensity range in 16 different colors for the selected channel.	Low High

Thumbnailⁱ Minatures of all available images. The checkboxes can be used to select which three images to compare in the window above. All images are also clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected miniature image has an orange overlay.	Antibodyⁱ Antibody used for analysis. Clicking the antibody ID links to the antibody validation page.	Cell lineⁱ Cell line used for analysis. Read more about the cell lines in the Human Protein Atlas.	BAC idⁱ BAC cell line used in the analysis. BAC cell lines have been obtained from the Hyman lab. The untransfected HeLa control cells are referred to as "normal".	Tagⁱ The GFP construct can be tagged to the target protein at either the N- or C-terminal, for some genes both versions are available.	Location antibody stainingⁱ Observed subcellular location(s) annotated for HPA-antibody targeting the endogenous protein.	Location tagged proteinⁱ Observed subcellular location(s) for the antibody targeting the GFP-tagged version of the protein.
	HPA004167	HeLa	5950	N-terminal	Vesicles	Vesicles
	HPA004167	HeLa	Normal	-	Vesicles	-

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg Foundation.

contact@proteinatlas.org