Specific expression of L1 cell adhesion molecule (gene: L1CAM) in collecting duct cells in the kidney is highlighted with multiplex immunohistochemistry (mIHC/IF).

The collecting duct is the final portion of the tubular system within the kidney. It receives and processes urine from multiple nephrons before moving it out of the kidney. The mIHC/IF kidney panel makes it possible to clearly distinguish the localization of proteins between different tubular structures within the kidney. Using mIHC/IF, L1CAM - a membrane glycoprotein involved in cell adhesion - is localized (green) to the cell membrane of the collecting duct cells (cyan). Other visualized kidney structures include proximal (white) and distal (red) tubules as well as endothelia (magenta).

In accordance with the spatial expression pattern, single-cell RNA sequencing data for L1CAM shows enhanced expression in collecting duct cells.

For more details about the mIHC/IF technique visit our method page.